기술이전

 

알파태아단백질의 절대정량을 위한 동위원소 질량분석법

관리번호
RS05202011200001
첨부파일
개발년도
2020
저자정보
유종신
참고문헌
Anal. Chem. 2020, 92, 12588−12595
관련장비
초고속 Q-TOF 질량분석기
응용분야
알파태아단백질의 절대정량을 통한 간암진단법
연구분야
BT
분석/적용분야
BT
기술분야
BT
분석/공동연구지원
알파태아단백질의 절대정량분석지원
요약
We developed an endoglycosidase-assisted absolute quantification (AQUA) method by which to measure N-glycosylated AFP levels in serum using liquid chromatography-parallel reaction monitoring with immunoprecipitation.
키워드
알파태아단백질, PRM, 동위원소, 절대정량 Alpha-Fetoprotein, Parallel Reaction Monitoring, Stable Isotope-LabeledN?Glycopeptide, Internal Stand
연구개발성과
혈액내 알파태아단백질의 동위원소 정량분석법개발
내 용
Alpha-fetoprotein (AFP) is a well-established serum biomarker for hepatocellular carcinoma (HCC) in clinical laboratories. However, AFP levels can often be high in benign liver diseases such as liver cirrhosis. For this reason, specifically, the level of the aberrant N-glycosylation of AFP has been proposed as a HCC biomarker to improve diagnostic performance using targeted mass spectrometry (MS). In this study, we developed an endoglycosidase-assisted absolute quantification (AQUA) method by which to measure N-glycosylated AFP levels in serum using liquid chromatography-parallel reaction monitoring with immunoprecipitation.